Application

ALT Activity Assay kit has been used to determine the activity of alanine aminotransferase (ALT) in serum samples.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is a pyridoxal-phosphate-dependent enzyme that catalyzes the reversible transfer of an amino group from alanine to A-ketoglutarate, generating pyruvate and glutamate. ALT is found primarily in liver and serum, but occurs in other tissues as well. Hepatocellular injury often results in an increase of serum ALT levels and serum ALT levels can be used as a marker for liver injury.

Principle

The ALT Activity Assay Kit provides a simple and direct procedure for measuring ALT activity in a variety of biological samples.ALT activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the pyruvate generated. One unit of ALT is defined as the amount of enzyme that generates 1.0 µmole of pyruvate per minute at 37 °C.

Suitability

Suitable for the measurement of alanine aminotransferase (ALT) activity in biological samples including tissue, cells and serum.